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MAbPac SEC-1 單克隆抗體分析柱戴安離子色譜柱耗材美國(guó)戴安Dionex
MAbPac SEC– 1 for Monoclonal Antibody (MAb) Analysis
MAbPac? SEC-1 is a size exclusion chromatography (SEC) column designed for monoclonal antibody (MAb) analysis, including monomers, aggregates, and fragments, under non-denaturing conditions, and in both high- and low-salt phases and volatile eluents.
? Proprietary hydrophilic bonded layer results in minimal non-desired interactions between the biomolecules and the stationary phase.
? Nonmetallic and Biocompatible PEEK? housing eliminates metal contamination from the column hardware.
? Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Corona? CAD detection.
? Rugged, reproducible column.
? Superior performance for the analysis of monoclonal antibodies, even at low salt concentrations.
Applications
SEC is frequently used because of its simple operating principle and features not found in other separation techniques including separating aggregates that are not easily distinguished by other chromatographic means. MAbs produced from mammalian cell culture may contain significant amounts of dimers and higher-order aggregates. The MAbPac SEC-10 is a high-resolution SEC column that is suitable for use with a wide range of biomolecules, including monoclonal antibodies and their aggregates.
| MAbPac? SEC-1 Monoclonal Antibody Analysis Column Specifications | |
|---|---|
| Column Dimension | 4 × 300 mm, 4 × 50 mm, PEEK? housing 7.8 x 300mm, 2.1 x 300mm, 2.1 x 150mm SST housing |
| Particle Size | 5 μm |
| Bonding Chemistry | Proprietary diol |
| Silica Substrate | Spherical, high-purity porous silica |
| Pore size | 300 ? |
| Flow Rate Range: | 0.76 to 1.0 mL/min: 7.8 mm ID columns 0.2 to 0.3 mL/min: 4.0 mm ID columns 0.05 to 0.075 mL/min: 2.1mm ID columns |
| Pressure Limit | < 1,000 psi: 7.8 mm x 300 mm < 600 psi: 4.0 x 150 mm <200 psi: 4.0 x 50 mm <1,000 psi: 2.1 x 300 mm < 600 psi: 2.1 x 150 mm |
| Temperature Range: | Ambient to 25 ?C |
| pH Range | 2.2–7.5 |
| Typical buffers: | 50 mM phosphate buffer (pH 6.8) + 0.3 M NaCl, PBS and other commonly used buffers |
| Solvent Compatibility: | Compatible with 100% organic solvents |
| Separation range for globular proteins | 10,000–1,000,000 |
| Exclusion limit for globular proteins | >1,000,000 |
| Detergent Compatibility | Compatible with 0.1% SDS (though binding will be irreversible and it is recommended that the column be dedicated to this application) |
The MAbPac SEC-1 column is based on high-purity, spherical, porous (300 ?), 5 μm silica particles covalently modified with a proprietary diol hydrophilic layer. The column is packed into nonmetallic, biocompatible, PEEK column housing to eliminate compromised chromatographic due to metal from the column hardware.
| Analytical Columns | |
|---|---|
| MAbPac SEC-1, 5μm 300 ?, analytical column (7.8 x 300mm) | 088460 |
| MAbPac SEC-1, 5μm, 300?, analytical column PEEK (4.0 x 300mm) | 074696 |
| MAbPac SEC-1, 5μm, 300?, analytical column PEEK (4.0 x 150mm) | 075592 |
| MAbPac SEC-1, 5μm 300 ?, analytical column (2.1 x 300mm) | SP6937 |
| MAbPac SEC-1, 5μm 300 ?, analytical column (2.1 x 150mm) | SP6938 |
| Guard Columns | |
|---|---|
| MAbPac SEC-1, 5μm, 300?, guard column PEEK (4.0 x 50mm) | 074697 |
MAbPac SEC-1 單克隆抗體分析柱戴安離子色譜柱耗材美國(guó)戴安Dionex，
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